Aquarius Spectrophotometers Have
Superb Versatility
Micro Sampling | Kinetics | PC Data Manipulation | Band Quantification
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Micro - Sipette Sampling
Small samples or multiple samples are conveniently measured using
the micro-sipette system.
Samples as small as 300µl may be measured in a 10mm pathlength cell
with very low cross contamination.
All measurements are made in the same cell, enhancing accuracy.
The pump, which is mounted in the sample compartment, operates
under the control of the instrument's micro-processor system.
Ultra-Micro Sampling
Valuable samples, such as biological samples prepared with costly
reagents, may be measured using a special ultra-microcell and holder.
With the system, samples of 50µl or even less may be accurately measured. |
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Reaction Kinetics
Both single cell and multi cell Kinetics are available with reaction
plots to screen and printer. Regression analysis is applied to the
user selected portion of reaction curves and results are reported
in Units/Litre. Data may be reprocessed as required and curves stored.
Sample temperature control is available for single cells and four
and six cell changers. |
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Advanced Kinetics
An example of advanced kinetics is shown below where time based
reaction data generated by the spectrophotometer, has been transferred
into Grafit, fitted to a curve and transformed into a more suitable
format for mathematical interpolation and calculation. In this particular
example, the basic data plot can yield the limiting rate (Vmax),
the Michaelis constant (Km) and the initial linear portion (Vmax/Km).
This has been transformed into the Eadie-Hofstee format and plotted
to give better precision in the calculation of these important factors
and constants.
Other transformations are available such as Lineweaver-Burk and
the Hill plot for allosteric enzyme systems.
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PC Data Manipulation
Data may be transferred to a PC for use with spread sheets and
other mathematical software packages.
More advanced reaction kinetics data manipulation may be readily
performed with Grafit software. Linear, non-linear or polynomial
curve fitting is easily accomplished together with non-linear regression
analysis. A wide selection of standard equations are provided, together
with an equation editor for customised equation fitting.
Data may also be transformed into other mathematical formats for
plotting and calculation. |
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Band Quantification
Accurate quantitative assays using band area or height, corrected
for background absorption, are exceptionally easy to carry
out with the Series 7000 Aquarius instruments.
Analytical results are displayed on the screen with construction
lines enabling the quality of the parameters to be assessed. |
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The procedure may be stored as a method with
security code protection in the integral memory of the instrument.
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