List of Articles in the Category HPLC Flow Meter

Simplified Validation of HPLC Pump Systems

Consistent and accurate performance is an absolute necessity to meet U.S. Food and Drug Administration (FDA) and other regulations regarding installation qualification (IQ), operational qualification (OQ) and performance qualification (PQ) of equipment used in the pharmaceutical and biopharmaceutical industries. An example is high-performance liquid chromatography (HPLC) pump systems used to identify analytes. The process demands accurate, reproducible column retention time as an analyte is pumped through the HPLC system.

Preventive maintenance is certainly important. In addition, regularly checking and confirming pump flow rate accuracy must be undertaken by periodic performance verification of the HPLC system.  Learn why the FlowCal 5000 digital liquid flowmeter simplifies what otherwise is a complex, time consuming operation.  Check out Bob Sandor’s article in the December 12, 2016, issue of Flow Control magazine.

How to Check Pump System Accuracy for HPLC Analysis

Digital HPLC Liquid Flow Meter At a Glance

Pump system accuracy is crucial for equipment used to conduct high performance liquid chromatography (HPLC) analysis in the pharmaceutical and other industries subject to regulatory oversight by the FDA and similar authorities. Among functions performed by HPLC are included checks for purity, monitoring changes in pharmaceutical intermediates during chemical synthesis scale-up, stability and dissolution tests, and performing ongoing quality control procedures.

A Brief on HPLC Analysis

Identifying an analyte by HPLC is based on accurate and reproducible column retention time as the analyte is pumped through an HPLC system.  Reproducible retention time is critical and depends on accurate, uniform, reproducible HPLC pump flow rates.

Note that it is important to distinguish between accuracy and precision.  These two terms may seem the same but they are not necessarily so.  For example, an HPLC system (or for that matter many other systems such as analytical scales) may test out as precise.  This … Read the rest

How Accurate is your HPLC Pump?

The FlowCal 5000 in its travel case

The FlowCal 5000 in its travel case

As researchers know a critical if not the most critical aspect of an HPLC pump system is providing uniform and reproducible column retention times as analytes are pumped through a liquid chromatography system.  This in turn relies on uniform and reproducible HPLC pump flow rates.  After initial operational qualification (OQ) periodic calibration checks of HPLC pump systems must be undertaken to confirm OQ and to identify and correct flow rate variances that may develop due to factors such as worn pump seals or sediments that interfere with flow rates.   These calibration tests are performed by checking flow rates while pumping solvent through the HPLC system.

HPLC Troubleshooting – the Hard Way and Easy Way

Several of our posts on the FlowCal 5000 digital liquid flow meter explain how the system works in comparison to manual methods.  Taking a paragraph from our Flow Meter Read the rest

Bottle-mounted FlowCal 5000 HPLC Flowmeter

How to Perform HPLC Calibration with a Digital HPLC Flowmeter

Bottle-mounted FlowCal 5000 HPLC Flowmeter

Bottle-mounted FlowCal 5000 HPLC Flowmeter

A CNNMoney article in August 2010 stated that recalls of prescription and over the counter drugs are surging, raising questions about the quality of drug manufacturing in the United States. The article mentioned FDA reports of more than 1,742 recalls in 2009 up from 426 in 2008. Because HPLC (high performance liquid chromatography) is frequently the method of choice for analyzing pharmaceutical compounds from research through development and manufacturing, HPLC systems should be regularly checked for consistency and accuracy by using a digital HPLC flowmeter. 

The role of factory-calibrated HPLC flow meters such as the FlowCal 5000 available from Tovatech is relatively simple but also highly precise.  It is used to identify malfunctioning valves and seals in HPLC flow systems that can lead to inaccurate formulations.  The FlowCal 5000 is a fast and accurate tool to support HPLC calibration and HPLC validation.  Record-keeping is simplified … Read the rest