List of Articles in the Category HPLC Flow Meter

How a Digital HPLC Liquid Flow Meter Works

HPLC Liquid Flow MeterBefore explaining how HPLC liquid flow meters work here’s an explanation of what digital HPLC flow meters do:  Digital HPLC flowmeters are used to measure the accuracy of High Performance Liquid Chromatography pumping systems in order to meet FDA and other regulations regarding installation qualification (IQ), operational qualification (OQ) and performance qualification (PQ) of equipment used in the pharmaceutical and biopharmaceutical industries as well as other analytical labs.

The process demands accurate, reproducible column retention time as an analyte (what is being analyzed) is pumped through the HPLC system.

These precision instruments are not to be likened to flow meters used on process lines measuring flow rates in, for example, thousands of gallons per minute but instead in fractions of milliliters per minute.

Why HPLC Pump System Accuracy is Important

As suggested above, meeting regulations (and protecting a company’s reputation) is the reason.  Consistent accurate performance is an absolute necessity. … Read the rest

Simplified Validation of HPLC Pump Systems

Consistent and accurate performance is an absolute necessity to meet U.S. Food and Drug Administration (FDA) and other regulations regarding installation qualification (IQ), operational qualification (OQ) and performance qualification (PQ) of equipment used in the pharmaceutical and biopharmaceutical industries. An example is high-performance liquid chromatography (HPLC) pump systems used to identify analytes. The process demands accurate, reproducible column retention time as an analyte is pumped through the HPLC system.

Preventive maintenance is certainly important. In addition, regularly checking and confirming pump flow rate accuracy must be undertaken by periodic performance verification of the HPLC system.  Learn why the FlowCal 5000 digital liquid flowmeter simplifies what otherwise is a complex, time consuming operation.  Check out Bob Sandor’s article in the December 12, 2016, issue of Flow Control magazine.

How to Check Pump System Accuracy for HPLC Analysis

Digital HPLC Liquid Flow Meter At a Glance

Pump system accuracy is crucial for equipment used to conduct high performance liquid chromatography (HPLC) analysis in the pharmaceutical and other industries subject to regulatory oversight by the FDA and similar authorities. Among functions performed by HPLC are included checks for purity, monitoring changes in pharmaceutical intermediates during chemical synthesis scale-up, stability and dissolution tests, and performing ongoing quality control procedures.

A Brief on HPLC Analysis

Identifying an analyte by HPLC is based on accurate and reproducible column retention time as the analyte is pumped through an HPLC system.  Reproducible retention time is critical and depends on accurate, uniform, reproducible HPLC pump flow rates.

Note that it is important to distinguish between accuracy and precision.  These two terms may seem the same but they are not necessarily so.  For example, an HPLC system (or for that matter many other systems such as analytical scales) may test out as precise.  This … Read the rest

How Accurate is your HPLC Pump?

The FlowCal 5000 in its travel case

The FlowCal 5000 in its travel case

As researchers know a critical if not the most critical aspect of an HPLC pump system is providing uniform and reproducible column retention times as analytes are pumped through a liquid chromatography system.  This in turn relies on uniform and reproducible HPLC pump flow rates.  After initial operational qualification (OQ) periodic calibration checks of HPLC pump systems must be undertaken to confirm OQ and to identify and correct flow rate variances that may develop due to factors such as worn pump seals or sediments that interfere with flow rates.   These calibration tests are performed by checking flow rates while pumping solvent through the HPLC system.

HPLC Troubleshooting – the Hard Way and Easy Way

Several of our posts on the FlowCal 5000 digital liquid flow meter explain how the system works in comparison to manual methods.  Taking a paragraph from our Flow Meter Read the rest