Bleomycin Separation

This illustrates the chromatographic separation of Bleomycin sulphate, a mixture of cytotoxic glycopeptide antibiotics used in the treatment of various types of carcinoma.

The USP assay procedure defines a 60 minute binary gradient followed by an isocratic elution.
The separation can be greatly accelerated using a much steeper gradient (30 min) followed by the isocratic elution.