Choosing a Laboratory Freezer for Enzyme Storage

Written by Rachel Kohn

Enzymes are the catalysts of the biochemical reactions that comprise metabolism; they do their job without changing themselves in the process.    Enzymes are responsible for bringing about almost all of the chemical reactions in living organisms. Without enzymes, these reactions take place at a rate far too slow for the pace of metabolism.*

Enzymes being studied by researchers must be stored in a enzyme freezer at temperatures in the region of -10 to -25˚C (+14 to -13˚F).  When stored at higher temperatures enzymes gradually denature and lose catalytic activity.  Proper storage conditions are essential to ensure that the enzymatic activity on the label is indeed the activity inside the bottle.   I have many not so fond memories of failed experiments in graduate school because the rather costly purified enzyme suspensions failed to perform as expected, all due to inadequate storage. 

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